《植物生理学报》 2010, 46(2): 150-152

盐胁迫下过量表达腺苷甲硫氨酸合成酶基因的转基因烟草的生长

戚元成* , 王菲菲

河南农业大学生命科学学院, 郑州450002

通信作者：戚元成；E-mail: qiyuancheng@yahoo.com.cn；Tel: 0371-63555153

摘　要：

以转腺苷甲硫氨酸合成酶基因(SsSAMS2)烟草纯合子 ST8-9 为实验材料, 研究盐胁迫下过量表达 SsSAMS2 对转基因 烟草生长影响的结果表明, 200 mmol•L-1 NaCl处理后的转基因烟草的光合速率和生物量都比野生型烟草高, 积累的自由多 聚胺比较多, 同时精氨酸脱羧酶的转录本也更丰富。显示转基因烟草的耐盐性比野生型烟草高, 多聚胺在烟株缓解盐胁中 可能是起了重要作用。

关键词：腺苷甲硫氨酸合成酶; 盐胁迫; 多聚胺; 转基因烟草

收稿：2009-11-09   修定：2009-12-18

资助：国家“863” 计划(2002AA629080)。

The Growth of Transgenic Tobacco Over-Expressing S-adenosylmethionine synthetase under Salt Stress

QI Yuan-Cheng*, WANG Fei-Fei

College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China

Corresponding author: QI Yuan-Cheng; E-mail: qiyuancheng@yahoo.com.cn; Tel: 0371-63555153

Abstract:

The effect of S-adenosylmethionine synthetase overexpression on the growth of transgenic tobacco under salt stress was studied by using homozygous ST8-9, transgenic tobacco plants over-expressing Suadea salsa S-adenosylmethionine synthetase (SsSAMS2). The transgenic plants showed higher photosynthetic rate, biomass, polyamine (PA) content and abundance arginine decarboxylase (ADC) transcription than wild-type (WT) plants under 200 mmol·L^-1 NaCl treatment. These results indicated that transgenic plants were more tolerant to salt stress than WT plants and PAs might play an important role in attenuating salt stress.

Key words: S-adenosylmethionine synthetase; salt stress; polyamine; transgenic tobacco